miR-484 targeting of Yap1-induced LPS-inhibited proliferation, and promoted apoptosis and inflammation in cardiomyocyte

ABSTRACT Apoptosis and inflammation were the main hallmarks of sepsis-induced cardiomyopathy (SIC). Yes-associated protein isoform 1 (Yap1) miR-484 involved in mitochondrial fission apoptosis, especially proapoptotic roles SIC. Here, we investigated role Yap1 lipopolysaccharide (LPS)-treated H9c2 cells. was downregulated, while elevated by LPS treatment. Cell counting kit-8, flow cytometry, western blotting, ELISA showed that inhibitor significantly improved cell viability, decreased suppressed NLRP3 inflammasome formation, reduced secretion inflammatory cytokines TNF-α, IL-1β, IL-6. Yap1, directly targeted shown luciferase assay, more like a compensatory regulator stimulation. Knockdown inverted effects inhibitor, including promoted apoptosis inflammation. These revealed mRNA to inhibit promote LPS-treated